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level: aiims questions

Questions and Answers List

level questions: aiims questions

Question	Answer
restriction endonucleases are used as	molecular knives for cutting DNA at specific sites
c-DNA probes are copied from the messenger RNA molecules with the help of	reverse transcriptase
in this procedure transient pores are made in the cell membrane to introduce gene constructs	electroporation
the first step in the southern blot technique	digestion of DNA by restriction enzymes
the polymerase chain reaction technology was discovered by	karry mullis in 1984
after 4 PCR cycles how many DNA molecules are formed from one DNA template molecules	from a single DNA template molecules, it is possible to generate 2^n DNA molecules after n no. of cycles in polymerase chain reaction
why are plasmids used as vectors for inserting foreign DNA into a cell?	because they can carry a foreign DNA fragment when inserted into it .
which organism has found extensive use in genetic engineering	agrobacterium tumefaciens
these are endonucleases which cleave DNA at specific sites and these are produced by bacteria for their defense mechanism.	restriction enzymes
these are generated by inducing a foreign DNA into a cell and regenerating a plant from that cell	transgenic plants
which vector is used for cloning in higher organisms	retrovirus
(biochemistry) a long linear polymer found in the nucleus of a cell and formed from nucleotides and shaped like a double helix; associated with the transmission of genetic information	DNA polymerase
if a recombinant DNA bearing gene for ampicillin resistance if transferred into E. coli cells and the host cells are transferred onto the agar plates containing ampicillin what will happen.	the transformed receipient cell will grow and the non transformed cell would die
in the PCR technology the DNA is segment is replicated over a billion times. this is catalyzed why which enzyme	Taq polymerase
DNA polymerase remain active in which temperature	high temperature
cohen and boyer isolated an antibiotic resistance gene in which year	1972
restriction enzymes ECO RI cuts the DNA between bases G and A only when the sequence in DNA is	GAATTC
the mobile genetic element is	transporon
GAATTC is the recognition site for which restriction endonuclease	Eco RI I
which bioengineered bacteria is utilized for cleaning of marine oil slicks?	pseudomonas putida
at the scene of the crime. bloodstains are obtained, which part of blood is used for DNA profiling.	leucocytes
what is used in DNA recombinant technique	virus
the correct order of the process in PCR is	denaturation, annealing and extenstion