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Index
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IMMUNO AND SERO
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Chapter 1
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Safety in the Immunology-Serology Laboratory
level: Safety in the Immunology-Serology Laboratory
Questions and Answers List
level questions: Safety in the Immunology-Serology Laboratory
Question
Answer
Name the federal or national agencies responsible for safety issues;
Safety Standards and Agencies
Describe the practice of Standard Blood and Body Fluid Precautions.
Prevention of Transmission of Infectious Diseases
Explain the proper handling of hazardous material and waste management, including infectious waste, chemicals, and radioactive waste.
Safe Work Practices for Infection Control
Selection and Use of Gloves; Facial Barrier Protection and Occlusive Bandages; Laboratory Coats or Gowns as Barrier Protection
Protective Techniques for Infection Control
Explain the importance and efficacy of handwashing.
Handwashing
Describe the importance of handling the specimens properly when it is transported.
Specimen-Processing Protection
Enumerate some other certain procedures used in medical laboratory.
Additional Laboratory Hazards
Demonstrate the proper decontamination of a work area at the start and completion of work and after a hazardous spill.
Decontamination of Surfaces, Equipment, and Spills
Containers for Waste; Final Decontamination of Waste Materials
Disposal of Infectious Laboratory Waste
Immunization; Screening Tests; Postexposure Prophylaxis
Disease Prevention
Explain the importance of having knowledge about basic first aid and its procedures.
Basic First Aid and Procedures
The primary purpose is to ensure safe and healthful working conditions for every U.S. worker. To ensure that workers have safe and healthful working conditions, the federal government passed the Occupational Safety and Health Act of 1970 and, in 1988, expanded the Hazard Communication Standard to apply to hospital staff. Occupational Safety and Health Act regulations apply to all businesses by U.S. Department of Labor through OSHA
Occupational Safety and Health Administration (OSHA)
In 1991, OSHA mandated that all clinical laboratories must implement:
Chemical hygiene plan Exposure Control Plan Material Safety Data Sheet (MSDS)
A set of standard precaution methods that activates infection control. They are the basic level of infection control practices.
Safety Work Practices for Infection Control
generates a Standard Precaution which is an approach to infection control that prevents occupational exposures to bloodborne pathogens
Center for Disease Control and Prevention (CDC)
one of the leading causes of preventable death in hospitals every year.
Infection
Addresses factors related to the spread of infections within the healthcare setting including different practices on their prevention This includes policies and procedures used to minimize the risk of spreading infections
Infection Control
Type of glove that is providing more tactile sensitivity
Latex gloves
Type of glove that is made from PCV ( a petroleum-based film) The primary benefit of vinyl disposable gloves is that they’re inexpensive to manufacture
Vinyl gloves
Who is the father of handwashing? He introduced handwashing standards after discovering that the occurrence of puerperal fever could be prevented by practicing hand disinfection in Obstetrical Clinics.
Ignaz Semmelweis
also referred to as nosocomial or hospital infection - an infection occurring in a patient during the process of care in a hospital or other healthcare facility
Health Care Associated Infection (HCAI)
are the most common Vehicle to transmit healthcare associated pathogens
Hands
Specimens should be transported to the laboratory in a plastic leakproof bag while the carrier will always be wearing protective gloves no matter what type of biological specimen he or she is handling.
Specimen-Processing Protection
nondisposable equipment - soaking overnight in a dilute (1:10) bleach solution and rinsing with methyl alcohol and water before reuse.
True
Disposable glassware or supplies HAVE CONTACT WITH BLOOD - Should be autoclave or incinerated.
True
Where to put specimen when being transported?
Plastic leakproof bags
PPE used in handling specimen
Protective gloves
Used in many laboratories for the processing of blood specimens
Specially constructed plastic splash shields
It should be periodically decontaminated
Specially constructed plastic splash shields
Diluted household bleach prepared daily inactives HBC in __ minutes and HIV in ___ minutes
10 and 2 minutes
It is the recommended disinfectant
Sodium chloride (Household bleach)
Areas contaminated by blood or other body fluids needs to be treated as ___ and requires prompt removal and surface ___
POTENTIALLY HAZARDOUS and disinfection
Factors to consider before disinfection 1. Different ___ 2. Different type of ___ 3. ___ of the surface 4. ___ of the spills
1. Strategies 2. Spills 3. Porosity 4. Volume
Decontaminate____ by soaking overnight in dilute bleach solution and rising with methyl alcohol and water before use
Nondisposable equipment
____ or ____ that have come into contact with blood should be autoclaved or incinerated
Disposable glassware or supplies
Autoclavew are recommended compared to incineration because it produces significantly ______
less air pollutants than incinerator
The process of burning hazardous materials at temperature high enough for a specific period of time to destroy contaminant
Incineration
Government agencies that regulates the control of infectious, chemical, and radioactive waste
OSHA and FDA