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level: BASIC SEROLOGIC TECHNIQUES

Questions and Answers List

level questions: BASIC SEROLOGIC TECHNIQUES

Question	Answer
HAS LED TO HIGHLY SPECIFIC AND SENSITIVE TESTING	MONOCLONAL ANTIBODY
MUST BE A COMPLETE DOCUMENT OF CURRENT TECHNIQUES AND APPROVED POLICIES AND SHOULD BE AVAILABLE AT ALL TIMES FOR ALL THE PERSONNEL AND IMPORTANT FO THEM TO REVIEW AT ALL TIMES	PROCEDURES MANUAL
PROCEDURES MANUAL: THESE DOCUMENTS MUST BE KEPT FOR ____YEARS REMOVALS OF THESE MANUALS MUST BE DOCUMENTED BY ASSIGNED INDIVIDUAL	5
____ OR TUBE CLOTTED AND RETRACTED AT ROOM TEMPERATURE.	GOLD OR RED TOP
CLOTTED BLOOD SHOULD BE REAMED WITH ____ AND SERUM HAVE TO BE ____	APPLICATOR STICK AND REMOVED
EXCESSIVE HEAT MAY CAUSE TO DETERIORATE THE _____ PRESENT	PROTEINS
_____CONTAMINATION MAY ALTER TEST RESULTS	BACTERIAL
____ FOR SPECIMENS WITHIN 24 HRS, -20C FOR SPECIMENS MORE THAN ___ HRS	2-8C AND 72
REPEATED ___ DETERIORATE THE SERUM	THAWING
MOST RECOMMENDED SPECIMEN FOR SEROLOGIC TESTING IS ?	ANTICOAGULATED BLOOD TO EXTRACT SERUM.
LIPEMIC, HEMOLYSED AND CONTAMINATED CAN BE ____	UNACCEPTABLE
_____ SHOULD BE PREVENETED, THEREFORE BLOOD EXTRACTION SHOULD BE DONE BEFORE MEAL AS MUCH AS POSSIBLE	CHYLOUS SPECIMEN
TESTS FOR ____ IN URINE MUST CAREFULLY FOLLOW THE PROCEDURE LIKE THOROUGHLY CLEANING OF GENITALIA TO PREVENT BACTERIAL CONTAMINATION	HCG (PREGNANCY TEST)
____IS KNOWN TO CAUSE LYSIS AND AGGLUTINATION	COMPLEMENT
HEATING THE SERUM___ FOR ___MINS WILL INACTIVATE THE COMPLEMENT PROTEIN (e.g. C1q)	@56C FOR 30
IF A TEST DELAY OF 4 HOURS OCCURS, SERUM CAN BE REINACTIVATED FOR ___ MINS @____C	10 Minutes @ 56
PIPETTES ARE ESSENTIAL IN IMMUNOLOGY AND SEROLOGY LAB	True
THEY ARE NEEDED TO TRANSFER SERUM FROM ONE CONTAINER TO ANOTHER CONTAINER.	PIPETTES
IT IS ALSO USED FOR TRANSFER OF REAGENTS	PIPETTES
____ ARE NOW MOST COMMONLY USED TYPES OF PIPETTE IN LAB	AUTOMATED PIPETTES
TYPES OF MANUAL PIPETTES	1. SEROLOGIC 2. OSTWALD 3. VOLUMETRIC
________ REPLACED MOSTLY THE MANUAL GLASS PIPPETES AS IT IS MORE ACCURATE AND EASIER TO USE.	CALIBRATED TRANSFER PIPETTES
A CURVATURE AT THE TOP OF THE LIQUID WHICH IS USED FOR READING THE VOLUME OF THE LIQUID.	MENISCUS
ALLOWS FAST AND REPETITIVE MEASUREMENTS AND DELIVERY OF SOLUTIONS IN EQUAL VOLUMES.	AUTOMATIC PIPETTE
THESE PIPETTES CAN BE AUTOMATIC OR SEMIAUTOMATIC THAT USES PISTON-OPERATED PLUNGERS TO INTAKE AND EJECT LIQUIDS THROUGH AIR PRESSURE.	AUTOMATIC PIPETTE
MOST COMMONLY USED IN LABORATORY MORE RELIABLE PREVENTS CONTAMINATION FASTER PACE TAKES TIME TO MASTER MAY BE CLOGGED WITH MATERIALS EXPENSIVE AND HARDER TO REPLACE/REPAIR	AUTOMATIC PIPETTE
USED FOR REPETITIVELY ADDING THE SAME AMOUNT OF VOLUME THIS DISPENSERS HAVE SETTING TO ADJUST THE AMOUNT OF VOLUME INTENDED TO USE	AUTOMATIC DISPENSERS
POINT TO NOTE IS THAT THE ______ FOR SEROLOGIC DETECTION OF ANTIBODY IS NECESSARY	TIMING OF TESTING
THE TIME WHEN THE DISEASE IS FIRST DISCOVERED OR DETECTED.	ACUTE PHASE-
USUALLY OCCURS 2 WEEKS LATER AFTER ONSET OF INFECTION	CONVALESCENT PHASE-
THIS IS THE RECIPROCAL OF THE HIGHEST DILUTION OF THE PATIENT SERUM IN WHICH THE ANTIBODY IS STILL DETECTABLE	ANTIBODY TITER
CAN BE MEASURED SEMIQUANTITATIVELY THROUGH SERIAL DILUTION OR QUANTITATIVELY THROUGH THE USE OF AUTOMATED MACHINES.	ANTIBODY TITER
THIS IS THE TOTAL AMOUNT OF ANTIBODY TESTED SPECIFICALLY WITH ITS ANTIGEN.	ANTIBODY TITER
IT IS DEFINED AS LABORATORY ASSAYS PERFORMED NEAR THE PATIENT (BEDSIDE) ALSO THIS INCLUDES TEST KITS AND HANDHELD MONITORS AT HOME	POINT-OF-CARE TESTING (POCT)
POINT-OF-CARE TESTING (POCT) ADVANTAGE	RAPID TEST RESULTS
POINT-OF-CARE TESTING (POCT) DISADVANTAGE	COST, QUALITY OF TEST, MONITORING
THESE ARE TESTS WITH LITTLE CHANCE OF NEGATIVE OUTCOMES WHEN PERFORMED INACCURATELY	WAIVED TESTS-
USUALLY AUTOMATED BUT MORE COMPLEX THAN WAIVED TESTS	MODERATELY COMPLEX TEST-
USUALLY NONAUTOMATED OR COMPLICATED TESTS THAT REQUIRES CONSIDERABLE JUDGEMENT	HIGHLY COMPLEX TEST-
EXAMINATION OF SLIDES PREPARED BY TECHS FROM FRESHLY COLLECTED SAMPLE	PROVIDER-PERFORMED MICROSCOPY TEST
QUALITY CONTROL STANDARDS: ALL LABORATORIES MUST MEET THE ____ REGARDLESS OF WHERE IT IS PERFORMED. IN PH CASE, DOH SET THESE STANDARDS AND ALLOWS MULTIPLE FACILITIES TO DO SUCH ASSESSMENTS. MONITORING OF TEST KITS ARE ALSO REQUIRED I____.EG HIV, HBSAG	QUALITY STANDARD INTERNATIONALLY
MOST COMMONLY USED TEST FOR THIS IS PREGNANCY TEST	NON-INSTRUMENT-BASED TESTING
A TEST STRIP SPECIFIC FOR HCG IS ADDED WITH SAMPLE (URINE) IN A TEST WELL. HCG PRESENT IN SAMPLE TRAVEL ACROSS THE REACTION PAD AND CAPTURED BY MONOCLONAL ANTI-HCG DYE ATTACHED IN THE PAD. COMPLEX ANTI-HCG THEN NOW FLOW INTO THE RESULT WINDOW WHERE IT IS CAPTURED BY IMMOBILIZED ANTI-HCG COMPLEX (RABBIT) IN “TEST REGION” ANOTHER AREA CALLED THE CONTROL REGION IS ATTACHED WITH IMMOBILIZED ANTIBODY TO CAPTURE ANTI-HCG DYE (WITH OR WITHOUT A COMPLEX)	IMMUNOCHROMATOGRAPHIC ASSAY/ LATERAL FLOW TEST
IT IS A HORMONE PRODUCED BY SYNCYTIOTHROPOBLASTIC CELLS	HUMAN CHORIONIC GONADOTROPIN
____ACTS AS STIMULANT TO CORPUS LUTEUM TO PRODUCE PROGESTERONE AND MAINTAIN THE PREGNANCY.	HUMAN CHORIONIC GONADOTROPIN
CAN ONLY BE FOUND IN LARGE QUANTITIES IN EARLY PREGNANCY AND CAN BE FOUND IN MALES TOO! WHEN HE HAS A TESTICULAR CANCER	HUMAN CHORIONIC GONADOTROPIN
HCG ALTHOUGH PRESENT IN PREGNANT WOMAN, MAY TEST NEGATIVE WHEN THE AMOUNT OF TSH AND LH ARE SIGNIFICANTLY HIGHER THAT NORMAL.	CROSS REACTIVITY
HCG ALTHOUGH PRESENT IN PREGNANT WOMAN, MAY TEST NEGATIVE WHEN THE AMOUNT OF ___ AND ___ARE SIGNIFICANTLY HIGHER THAT NORMAL.	TSH AND LH