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Index
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MCPH 4
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BACTERIA
»
DIAGNOSTIC TESTS
level: DIAGNOSTIC TESTS
Questions and Answers List
level questions: DIAGNOSTIC TESTS
Question
Answer
Coagulase Test (+) Catalase Test (+) Dnase TEST (+)
Staphylococcus aureus
Coagulase Test (-) Catalase Test (+) Dnase TEST (-)
Staphylococcus epidermidis and Staphylococcus saprophyticus
Catalase test (-) PYRase test (+) Bile esculin test (-)
Streptococcus pyogenes
Catalase (-) CAMP Test (+) PYRase (-) Bile esculin Test (-) Na Hippurate hydrolysis Test (+)
Streptococcus agalactiae
CAMP test (-) PYRase (-) Bile esculin test (-)
Streptococcus equisimilis
CAMP test (-) PYRase (-) Bile esculin test (+)
Streptococcus equinus and Streptococcus bovis
CAMP test (-) PYRase (+) Bile esculin test (+)
Enterococcus faecalis and Enterococcus durans
CAMP test (-) PYRase (-) Bile esculin test (-) Mouse virulence test (-)
Streptococcus mutans and Streptococcus constellatus
Capsular swelling test (+) Mouse virulence test (+)
Streptococcus pneumoniae
Oxidase test (+) Catalase test (+)
Neisseria gonorrhoeae and Neisseria meningitidis
Diagnostic tests: Lecithinase (+) only
Bacillus cereus
String of pearls reaction (+) Ascoli precipitation test (+) Lecithinase (+)
Bacillus anthracis
Diagnostic tests: Toxigenecity test (+) modified Elek test (+) Schick test (Diphtheria)
Corynebacterium diptheriae
Diagnostic tests: Catalase (+) oxidase (+) CAMP test (+) Bile esculin hydrolysis (+) Hydrogen sulfide (-) Ocular Anton Test (Listeriosis)
Listeria monocytogenes
Diagnostic tests: Catalase (-) Hydrogen sulfide (+)
Erypsipelothrix rhusiopathiae
Diagnostic tests: Lecithinase (-) Gelatinase hydrolysis (+)
Clostridium tetani
Diagnostic tests: Lecithinase (-) Lipase (+)
Clostridium botulinum
Diagnostic tests: Lecithinase (+) Lipase (-) Stormy fermentation of milk (+) Nagler reaction or lecithinase test (+) CAMP test (+)
Clostridium perfringens
Diagnostic tests: Lecithinase (-) Lipase (-) Gelatinase hydrolysis (+)
Clostridium difficile
A test used to differentiate the Staphylococcus aureus from the rest of the Staphylococcus species.
Coagulase Test
Test used to differentiate the Staphylococci from Streptococci.
Catalase Test strepto - negative staph - positive
It is widely used to differentiate enterococci and non-enterococci group D streptococci.
Bile esculin Test positive: can hydrolyze esculin to esculetin
A colorimetric enzymatic test for the detection of the __
PYRase Test
Used to differentiate the Staphylococcus from other Staphylococci.
Dnase Test
A glycine conjugate of benzoic acid. The ability of microorganism to hydrolyze __ to glycine and benzoic acid
Na hippurate hydrolysis Test
known as the “Quelling Reaction”.
Capsular Swelling Test Swelling: Positive
Used for the presumptive identification of group B beta-hemolytic streptococci.
CAMP Test
Test the ability of the agent to produce a disease by injecting it to a mouse
Mouse Virulence Test
Ability to produce certain cytochrome C oxidases.
Oxidase Test
Blood agar with penicillin
String of pearls reaction
uses tissue extract and anthrax antiserum
Ascoli precipitation Test
Test for an enzyme released by bacteria and they have the ability to destroy animal tissues
Lecithinase, Lecithinase test
1 Test particular for the scarlet fever 2 also a susceptibility test for scarlet fever ( it is also used to determine which antimicrobials will inhibit the growth of the bacteria or fungi which causes the specific infection)
1 The Schultz-Charlton 2 The Dick’s Test
a pathogenicity test particularly for listeriosis
Ocular Anton Test
a susceptibility test to diphtheria.
Schick Test
It results when there is an abundance in gas, such as Carbon dioxide and Hydrogen, that may be formed as a result of lactose fermentation
Stormy fermentation of milk