level: Level 2
Questions and Answers List
level questions: Level 2
| Question | Answer |
|---|---|
| Types/ Techniques of chromatography useful in qualitative and quantitative analysis that are employed in USP assays: | 1 Column 2 Gas 3 Paper 4 TLC 5 HPLC |
| ordinarily more useful for identification because of convenience and simplicity | Paper and TLC |
| THEORY OF CHROMATOGRAPHY: | 1 Plate theory 2 Rate theory |
| considers the CHROMATOGRAPHIC SYSTEM as series of discrete layers of THEORETICAL PLATES | Plate theory |
| considers the DYNAMIC OF THE SOLUTE PARTICLE as it passes thru the void spaces in the stationary phase | Rate theory |
| a measure of the FRACTION of its TOTAL ELUTION TIME that any compound spends in the mobile phase used in paper and TLC chrom | Rf Retardation factor |
| TIME THAT ELAPSES from the START of the chromatogram to the ELUTION MAXIMUM of the solute | Retention time (tr) |
| Rf formula | Rf = distance travelled by solute/ distance travelled by solvent |