level: Level 6
Questions and Answers List
PAPER CHROMATOGRAPHY
level questions: Level 6
| Question | Answer |
|---|---|
| STATIONARY PHASE: filter paper (the tightly bound water is the actual SP) Liquid-liquid partition chrom, although adsorption to cellulose also occurs | PAPER CHROMATOGRAPHY (PC) |
| paper chroma Paper impregnated w/ 20% - 40% formamide in ethanol is used - chloroform (hydrophilic substance) - benzene ( for subst. of medium polarity) - cyclohexane (for hydrophobic subst) - or mixtures of these | . |
| (hydrophilic substance) | chloroform |
| ( for subst. of medium polarity) | benzene |
| (for hydrophobic subst) | cyclohexane |
| paper chroma Sample Preparation and Applications: | Drugs applied to paper in soln. in volatile solvents such as ethanol, acetone or chloroform 3 -5 cm from lower edge in ascending development 3-8 mm diam – size of spot 2-3 cm apart – adjacent spots |
| 1 size of spot 2 adjacent spots | 1) 3-8 mm diameter 2) 2-3 cm apart |