Types of chromatography and what are their principles

-column chromatography which takes advantage of differences in protein charge, size, binding affinity, and other properties -Ion-exchange chromatography exploits differ- ences in the sign and magnitude of the net electric charge of proteins at a given pH -cation-exchange chromatography the solid matrix has negatively charged groups. In the mobile phase, proteins with a net positive charge migrate through the matrix more slowly than those with a net negative charge, -Size-exclusion chromatography, separates proteins according to size. In this method, large proteins emerge from the column sooner than small ones -Affinity chromatography is based on binding affinity