What are the Mechanisms Exist to Prevent Premature Reinitiation of Replication?

SeqA binds to hemimethylated DNA and is required for delaying rereplication. • SeqA may interact with DnaA. • As the origins are hemimethylated they bind to the cell membrane and may be unavailable to methylases. • The dat locus contains DnaA-binding sites that titrate availability of DnaA protein. • Hda protein is recruited to the replication origin to convert DnaA-ATP to DnaA-ADP.