What are the limitations of both X-rayDiffraction and NMRDistanceMeasurement?

• X-rayDiffraction ✓ Only a small number of proteins can be made to form crystals ✓ A crystal is not the protein’s native environment ✓ Very time consuming • NMRDistanceMeasurement ✓ Not all proteins are found in solution ✓ This method generally looks at isolated proteins rather than protein complexes ✓ Very time consuming