How does Isolation Influence the rising of New Species?

NEW SPECIES ARISING DUE TO ISOLATION: 1) two populations of a species can become geographically separated because of the environment 2) isolation can prevent interbreeding and the combination of genes within a species 3) different mutations can take place in the isolated groups and create different phenotypes within a particular location 4) over time species may evolve to be different to each other, and they will not be able to interbreed