METHODOLOGIES General Sample and Patient Consideration

Cholesterol values rise with age • 12 hrs fasting ; CMs almost completely cleared within 6 9hrs after food ingestion • Total cholesterol and HDL C generally don’t need for fasting • Plasma or serum can be used for only cholesterol , TAG , and HDL C are measured; LDL C is calculated Friedewald equation • Plasma should be separated from cells within 3 hrs • Plasma (EDTA) is preferred for lipoprotein measurement