Factors affecting rate of reaction

-enzyme concentration (run out substrates for es complexes) -substrate concentration increases so more es complexes then substrate conc is no longer the limiting factor ( max es complexes not enough enzymes) -temperature (kinetic energy increases so more collisions creating es complexes quicker until it reaches optimum temp and the max everything until it suceeds this then causes hydrogen/ionic bonds in enzyme to break Causes enzyme’s ts to change,so active s changes no longer compimentary) PH (ph changes cause hydrogen bonds to break in the specific tertiarty structure)