MBS1002 Biomedical Approaches: what is the principle of cell-based assay? and what is an example? what techniques are considered cell-based assays?

Question

what is the principle of cell-based assay? and what is an example? what techniques are considered cell-based assays?

Mirte Schattorie · Accepted Answer

cell-based assays assess the efficacy of compounds in a cellular environment, which aids to understaning of the cell.

1. plasmids that contain the gene of interest are engineerd.
2. the plasmid is transfected into the cells. 
3. the corresponding protein is expressed and validated. 
4. immunofluorescence is done to check for the presence of the autoantibody.

cell-based assays are; flow cytometry, immunocytochemistry, immunofluorescence on cells

MBS1002 Biomedical Approaches

What is the principle of cell-based assay? and what is an example? what techniques are considered cell-based assays?

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