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Unit 3 Biochemistry

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Question:

A plasmid was digested using restriction enzymes. The following DNA fragments were produced: 1200 bp, 1800 bp, 200 bp, and 750 bp. T/I C (a) Which fragment will travel the fastest in an agarose gel? Why? (b) Which fragment will travel the slowest in an agarose gel? Why? (c) Sketch the gel pattern that would result after running this plasmid digestion in an agarose gel.

Author: fahr dat



Answer:

(a) The 200 bp fragment will travel the fastest because it is the shortest. (b) The 1800 bp fragment will travel the slowest because it is the longest.


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fahr dat
fahr dat