What are the pros and cons of RNA sequencing?

Pros: high resolution (up to single bases), high throughput, low background noise, able to differentiate isoforms, able to distinguish allelic expression variants, required amount of RNA is low, can reveal SNPs or other mutations, can be used to identify new genomic sequences (novel information), can be used to map complex transcriptomes. cons: expensive (but relatively low cost for large mapping large transcriptomes), difficult to store, retrieve and process large amounts of data, time-consuming, reverse transcripton needed, complex to analyse.