Question:
What are characteristics of the optics component of the flow cytometer?
Author: Mirte SchattorieAnswer:
The optics focuses the light on the cells and transmits the scatter or fluorescence to the electrics. lenses are used to shape and focus the laser beam, which produces energized electrons in high energy states. when these electrons fall back, photons are produced and these photons deflect around the cell. the optics include a series of filters/dichroic mirrors that transmit part of the light, depending on the type of filter; - bandpass filters transmit a specific range of wavelengths. - short pass filters transmit wavelengths that are equal to or shorter than a specific wavelength. - long pass filters transmit wavelengths that are equal to or longer than a specific wavelength.
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