biotechnology: principals and processes
🇬🇧
In English
In English
Practice Known Questions
Stay up to date with your due questions
Complete 5 questions to enable practice
Exams
Exam: Test your skills
Test your skills in exam mode
Learn New Questions
Manual Mode [BETA]
The course owner has not enabled manual mode
Specific modes
Learn with flashcards
Complete the sentence
Listening & SpellingSpelling: Type what you hear
multiple choiceMultiple choice mode
SpeakingAnswer with voice
Speaking & ListeningPractice pronunciation
TypingTyping only mode
biotechnology: principals and processes - Leaderboard
biotechnology: principals and processes - Details
Levels:
Questions:
66 questions
| 🇬🇧 | 🇬🇧 |
| Restriction endonucleases are used as | Molecular knives for cutting DNA at specific sites |
| C-DNA probes are copied from the messenger RNA molecules with the help of | Reverse transcriptase |
| In this procedure transient pores are made in the cell membrane to introduce gene constructs | Electroporation |
| The first step in the southern blot technique | Digestion of DNA by restriction enzymes |
| The polymerase chain reaction technology was discovered by | Karry mullis in 1984 |
| After 4 PCR cycles how many DNA molecules are formed from one DNA template molecules | From a single DNA template molecules, it is possible to generate 2^n DNA molecules after n no. of cycles in polymerase chain reaction |
| Why are plasmids used as vectors for inserting foreign DNA into a cell? | Because they can carry a foreign DNA fragment when inserted into it . |
| Which organism has found extensive use in genetic engineering | Agrobacterium tumefaciens |
| These are endonucleases which cleave DNA at specific sites and these are produced by bacteria for their defense mechanism. | Restriction enzymes |
| These are generated by inducing a foreign DNA into a cell and regenerating a plant from that cell | Transgenic plants |
| If a recombinant DNA bearing gene for ampicillin resistance if transferred into E. coli cells and the host cells are transferred onto the agar plates containing ampicillin what will happen. | The transformed receipient cell will grow and the non transformed cell would die |
| In the PCR technology the DNA is segment is replicated over a billion times. this is catalyzed why which enzyme | Taq polymerase |
| DNA polymerase remain active in which temperature | High temperature |
| Restriction enzymes ECO RI cuts the DNA between bases G and A only when the sequence in DNA is | GAATTC |
| The mobile genetic element is | Transporon |
| At the scene of the crime. bloodstains are obtained, which part of blood is used for DNA profiling. | Leucocytes |
| The correct order of the process in PCR is | Denaturation, annealing and extenstion |
| Human genome project is closely associated with the rapid development of a new area in biology called as | Bioinformatics |
| What are palindromic base sequence | Palindromic sequences in DNA molecule are group of bases that forms the same sequence when read in both forward and backward direction |
| Plasmids are the extrachromosomal part in the bacteria and are useful in | Recombinant DNA technology |
| Plasmids are extra chromosomal material possesing single stranded DNA, they are present in | In eukaryotes as well as in prokaryotes |
| In recombinant DNA technology, recombinants DNA are usualy transferred to E.coli, yeat beacuse of their rapid multiplication | This technology is employed for combining DNA from two different organisms to produce recombinant DNA |
| Restriction enzymes cut the strand of DNA to produce sticky ends.(named so because they form h bond with theiir complemetary counter parts) | The stickness of the ends facilitates the action of the enzyme DNA ligase, not DNA polymerase. |
| Some features of plasmids | Independent replication, circular structure, transferable |
| The taq polymerase enzyme is obtained from | Thermus aquaticus |
| An analysis of chromosomal DNA using the southern hybridisation technique uses | Electrophoresis, blotting and autoradiology |
| The colonies of recombinant bacteria appears white in contrast to blue colonies of non-recombinant bacteria because of | Non-recombinant bacteria containing beta-galactosidase |
| Stirred tank bioreactors hav been designed for | Availability of oxygen throughout the process |
| Chromosomes in a bacterial cell can be 1-3 in number and | Are always circular |
| The plasmids present in bacterial cells are | Circular double helical DNA molecules |
| PCR and restriction fragment length polymorphism are the methods for | Genetic fingerprinting |
| At what temp. does the DNA polymerase used in PCR remain active | At high temperature |
| For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of | Gold or tungsten |
| Biolistics(gene gun) is suitable for | Constructing recombinant DNA by joining with vectors |
| The most likely reason for the development of resistance against pesticides in insect damaging a crop is | Random mutations |
| After completing transformation experiment involving the coding sequence of enzyme beta-galactosidase, the re-combinant colonies should | Not give blue colour |
| Which of the following method can be used for making the bacterial cell competent | Treating with specific conc. of divalent cation (Ca2+) heat shock |
| Uring isolation of DNA, addition of which of the following causes precipitation of purified DNA | Chilled ethanol |
| If we ligate a foreign DNA at the Bam H1 site of tetracycline resistance gene in pBR322 the recombinant plasmid will | Show ampicillin resistance only |
| Which can be used as vector for transfer of DNA segment | Plasmid and bacteriophage |
| PCR is use to | Amplify a small amount of DNA |
| What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis | The smaller the fragment size, the father it moves |
| Which of the following is not a feature of the plasmids | Single-stranded |
| The taq polymerase enzyme is obtained from | Thermus aquaticus |
| Stirred-tank bioreactors have been designed for | Availability of oxygen throughout the process |