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BIOLOGY TOPIC 3 GENETICS

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Question:

What is the process of autoradiography? (6) (hint: AgBr and RA)

Author: Sammy Universe



Answer:

1) cells grown in radioactive thymidine and is mixed with the DNA 2) cells are lysed to isolate chromosomes and embedded on a slide 3) placed in RA sensitive mixture of AgBr (silver bromide) 4) radiation from thymidine changes Ag+ = insoluble Ag 5) excess AgBr is washed away = leaving Ag (small black dots) 6) film develops to show chromosomal DNA


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