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Question:
Describe and Explain the Practical of Testing the Action of Antibiotics [Part 1: Explain up to when the Bacteria has Covered the Jelly]
Author: eric_galvaoAnswer:
1. Pour Hot, Sterilised Agar Jelly into a Petri Dish [Round, Small Dish] 2. When the Jelly Cools, Inoculating Loops [Wire Loops] used to Give Microorganisms to the Culture Medium [Agar Jelly] 3. Take 3 Discs of Filter Paper, and Soak 1 in Antibiotic A, Soak another in Antibiotic B and the Last One is a Control, Soaked in Sterile Water [Useful later] 4. Place the Discs onto the Jelly via Sterile Forceps, and Tape the Lid onto the Dish to not allow Contamination from other Microbes. Antibiotics will soak into the Agar Jelly [Diffuse] 5. Leave for around 2 Days at 25 Degrees. What you will see is the Bacteria will Multiply and make a Lawn.
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