Dam1/DASH complex experiment

The Dam1/DASH complex can harness the force generated by microtubule depolymerisation to move beads in vitro. In microtubules are attached to a glass slide and Dam1 complex covalently couple to a bead is added. Laser based optical tweezers are used to move the bead to assess the force that can be applied on the Dam1/DASH complex without it falling off shrinking microtubule ends.