MAP65

MAP65 bundles and stabilises anti-parallel MTs. This is especially important during for formation of a bi-polar spindle. MAP65 has both a MT binding domain and a dimerization domain. The configuration of MAP65 dimers allows only anti-parallel microtubules to interact. The EM image shows a cross-section through an anaphase B spindle. Serial sections through the entire spindle reveal that microtubules associate only with microtubules from the opposite pole in a stable squared packed structure. Bridges can be observed which may be MAP65 itself.