MBS1002 Biomedical Approaches: how can efficiency of transfection be assessed?

Question

how can efficiency of transfection be assessed?

Mirte Schattorie · Accepted Answer

- fluorescence microscopy with fluorescently tagged molecules
- real time PCR to directly measure nucleic acid expression level
- plasmid reporting system
- flow cytometry to quantify numbers of fluorescently labelled transfected cells
- immunostaining

MBS1002 Biomedical Approaches

How can efficiency of transfection be assessed?

- fluorescence microscopy with fluorescently tagged molecules - real time PCR to directly measure nucleic acid expression level - plasmid reporting system - flow cytometry to quantify numbers of fluorescently labelled transfected cells - immunostaining

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