How to produce an animal model for McArdle disease?

1. produce a recombination vector including the mutation. 2. make the vector into a linear DNA sequence. 3. introduce the vector into stem cells by electroporation to allow for cell permeabilization. 4. grow the cells (under neomycin selection). 5. identify the recombinant clones using PCR. 6. confirm that homologous recombination has occurred using southern blot; the lengths of the wild-type and mutant DNA should be different, as measured by a probe. 7. perform a karyotype analysis.