MBS1002 Biomedical Approaches: what are advantages and disadvantages of third generation sequencing techniques over NGS and Sanger sequencing?

Question

what are advantages and disadvantages of third generation sequencing techniques over NGS and Sanger sequencing?

Mirte Schattorie · Accepted Answer

advantages
- no amplification is needed, which prevents PCR bias. 
- haplotying can be done; the determination of haplotypes (DNA variants of a single chromosome that are inherited together) from unordered DNA.
- can generate very long sequences (200kb).

disadvantages
- higher error rate.
- higher cost per kb compared to NGS.

MBS1002 Biomedical Approaches

What are advantages and disadvantages of third generation sequencing techniques over NGS and Sanger sequencing?

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