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MBS1002 Biomedical Approaches

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Question:

What are examples of third generation sequencing techniques?

Author: Mirte Schattorie



Answer:

PacBio: wells that have a DNA polymerase attached to their bottom are used. fluorescence nucleotides are added to the wells and the unknown DNA strand is extended by a polymerase. each time a fluorecent nucleotide is added, a signal is emitted. while the strand is elongated, you know the sequence. Oxford nanopore: native DNA is pulled through a very small channel, for each nucleotide that exists the channel, a little current flows through and is detected. each nucleotide will produce a different current, by which the nucleotide can be identified.


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